Orthotopic Models – Realistic Tumor Microenvironments

Orthotopic models mimic the human disease state more accurately than subcutaneous models by implanting tumor cells or tissue directly into the organ of origin. This preserves the tumor’s interaction with local stroma, vasculature, and immune environment, making these models essential for studying invasion, metastasis, and drug response in a clinically relevant setting.

Why choose orthotopic models?

• Organ-specific tumor growth: Captures tissue- and site-specific drug responses
• Natural metastatic behavior: Enables observation of spontaneous or distant-site metastasis
• Higher predictive value: Better recapitulates clinical efficacy vs. subcutaneous or cell-line-only models
• Real-time tumor tracking: Bioluminescence and fluorescence imaging for dynamic monitoring of progression and response
• Supports rare & aggressive cancers: Includes brain metastases, hematologic malignancies, and pretreated tumors

At LIDE, we offer orthotopic models built on PDX, CDX, and hematological tumors, across a wide spectrum of solid and liquid cancers.

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Summary of available orthotopic PDX models

Leveraging our best-in-class PDX library, LIDE provides well-characterized orthotopic PDX models—each using luciferase-labeled tumor cells for non-invasive monitoring.

Analysis and Readouts:

• IVIS bioluminescent imaging for real-time tracking
• FACS and molecular profiling for endpoint validation
• Tumor burden, survival, and toxicity assessment

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Summary of available orthotopic CDX models

When patient tissue isn’t available or preferred, LIDE provides well-characterized orthotopic CDX models across multiple organs and tumor types.

Benefits of using orthotopic CDX Models:

• Enables rapid proof-of-concept and dose-response studies
• Supports mechanism-of-action and metastasis tracking
• Scalable and reproducible: ideal for early screening or validation before PDX scale-up

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Model Customization Available:

If your study requires a specific indication, strain, or delivery route, LIDE’s scientific team can support custom orthotopic development on request.

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Featured Studies