Syngeneic Mouse Models – Fast & Immunocompetent

Syngeneic models remain a cornerstone for immuno-oncology (IO) drug development, offering the speed, cost-efficiency, and immunological integrity needed for early-stage evaluation. LIDE’s syngeneic and humanized syngeneic mouse platforms are designed to evaluate immune checkpoint inhibitors, cytokine modulators, bispecific antibodies, cell therapies, and combination regimens in fully immunocompetent hosts.

Why Consider Syngeneic Models?
AdvantageImpact on R&D
Fully functional immune systemEnables evaluation of immune-mediated antitumor effects
Rapid tumor growthShort study timelines accelerate go/no-go decisions
Reproducible and scalableConsistent tumor take rates for high-throughput IO screening
Broad tumor repertoireSolid and hematologic malignancies represented
Compatible with genetic engineeringAllows knock-in/knock-out models for human target evaluation

LIDE’s Syngeneic Model Portfolio

We maintain a diverse collection of murine tumor cell lines covering solid, blood, and orthotopic models. All models are available with or without bioluminescent variants for real-time, non-invasive tumor monitoring.

  • Brain: GL261
  • Breast: 4T1 (subcutaneous & mammary fat pad), EMT-6
  • Colorectal: CT26.WT, MC38
  • Gastric: MFC
  • Kidney: RAG, Renca
  • Liver: H22, Hepa1-6
  • Lung: LLC1 (LL/2)
  • Pancreas: PAN02
  • Prostate: RM-1
  • B-cell Lymphoma: A20
  • T-cell Lymphoma: E.G7-OVA
  • Melanoma: B16-F10
  • MBT2

Orthotopic Syngeneic Models:

LIDE also offers orthotopic syngeneic models for greater clinical relevance by implanting tumors into their organ of origin. Available models include:

  • Liver: Hepa1-6-luc, H22-luc
  • Breast: 4T1-luc (triple-negative)
  • Pancreas: Panc02-luc
  • Bladder: MB49-luc

Humanized Syngeneic Models

For evaluating human-specific targets in an immunocompetent setting, we offer huPD-1 or huPD-L1 knock-in syngeneic models. This is perfect for testing antibodies like pembrolizumab or nivolumab, which do not cross-react with murine PD-1.

Model Customization Available

If your study requires a specific indication, strain, or delivery route, LIDE’s scientific team can support custom syngeneic model development on request.

Example Study:

Drug efficacy analysis in EMT-6 subcutaneous transplantation model after administration of anti-mPD-1 therapy. All 5 mice exhibited partial response. TGI (tumor growth inhibition) rate was 66.16%.

The breadth of LIDE’s IO platform supports studies for both adaptive and innate immunity, with fast syngeneic models for proof of concepts or humanized models for more robust validation.