High Throughput RNAi Screening

  • LIDE has 2 functional library screening platforms, which could discover new targets directly by evaluating the effect of the knockout/knockdown of specific genes. Genome-wide CRISPR/Cas9 screens have been powerful tools for large-scale identification of new targets for cancer and universal screening libraries for human or mouse genome are established and mature. Comparing to genome-wide CRISPR/Cas9 screens, arrayed pro-siRNA library targeting all individual genes expressed in target cells and screen for candidate genes involved in any phenotype.

Genome-wide CRISPR-Cas9
- Pro-siRNAs system utilizes the unique function of the p19 protein, which can bind to
  and stabilize ~21-nt double-stranded RNA species produced by endogenous RNase III in E. coli.
- Pro-siRNA contains many siRNA sequences targeting the same gene are non-toxic to human cells.
- The pro-siRNA screening strategy is to clone mRNAs, isolated from the target cells, into a
  suitable pro-siRNA producing plasmid to create a pro-siRNA plasmid library.
 

  • Potential functional genes can be analyzed by Functional Genomic Imaging (FGI) (Learn More
    Platform developed by LIDE. Besides FGI, LIDE also provides classic bioinformatics analysis for both genomics and transcriptomics data. According to the analysis of specific sample groups, for example, drug sensitive or resistant group, researchers could get biomarker or potential targets. 
    Functional Genomic Imaging (FGI)